anti human fc antibody hfc probe  (Gator Bio Inc)

Journal: mAbs

Article Title: Identification of novel anti-CD16a antibody clones for the development of effective natural killer cell engagers

doi: 10.1080/19420862.2024.2381261

Figure Lengend Snippet: Measurement of binding constant (K D ), association constant (K on ) and dissociation constant (K off ) of anti-CD16 antibody clones by BLI. (a) anti-CD16a clones in IgG1 format; (b) anti-CD16a in fab format, in the absence or presence of 10 mg/mL of hIgG; (c) anti-CD16a in monovalent BiKE format; (d) anti-CD16a in the bivalent IgG-like BsAb format; (e) anti-CD16a in the bivalent BiKE-IgG4 format. An anti-HIgG Fc biosensor was used to capture Fc-containing antibodies (a, d and e) or streptavidin biosensor was used to capture biotinylated CD16a (b and c). Non-biotinylated CD16a (a, d and e), CD16b (a), fab (b) or BiKE (c) at serial dilutions was added to the biosensors, in the presence (b) or absence (a, b, c, d and e) of 10 mg/mL of hIgG.

Article Snippet: A Gator Anti-HIgG Fc biosensor (Cat.: PL168–160003) was used to capture IgG1 antibody or IgG4 bivalent engager to detect its binding to CD16, or a streptavidin biosensor (Cat.: 160002) to capture the biotinylated CD16 antigen for detection of binding to monovalent antibodies, e.g., Fab, monovalent BiKE.

Techniques: Binding Assay, Clone Assay